Expressions of p34cdc2 and cyclin B1 in human cervical carcinoma and its clinical significance / 肿瘤

ABSTRACT

Objective: To study the expression of p34cdc2 and cyclin B1 in human cervical carcinoma, and the relationship between their expression and clinicopathologyical features of cervical cancer. Methods: A quantitative real-time reverse transcription polymerase chain reaction and Western blotting assay were used to analyze the expression levels of p34cdc2 and cyclin B1 mRNA and protein, respectively, in fresh invasive cervical cancer (n = 62) and control cervical tissues (n = 15). Results: The expression levels of p34cdc2 and cyclin B1 mRNA and protein were significantly higher in cancer tissues than those in control cervical tissues (P = 0.004, P = 0.013; P = 0.016, P = 0.029), and mainly displayed overexpression. Significant positive correlation was found between the expression of p34cdc2 and cyclin B1 mRNA (r = 0.527, P = 0.001) and protein (r = 0.432, P = 0.022) in cervical cancer tissues. A statistical significance was found between the expressions of p34cdc2/cyclin B1 mRNA and lymphatic metastasis in cervical cancer (P = 0.038, P = 0.001). No statistically significant association was found between the age, histological types, the differentiation degree, clinical stages and expression of p34cdc2/cyclin B1 (P > 0.05). Conclusion: p34cdc2 and cyclin B1 are important molecules in regulation of cervical carcinogenesis. Over-expression of p34cdc2/cyclin B1 stimulates cervical cancer cells to overcome G2/ M checkpoint and enter M phase in cell cycle. The high-expression of p34cdc2/ cyclin B1 might become a novel biomarker for studying the mechanism underlying the tumorigenesis and lymphatic metastasis of cervical cancer.