The effect of lysophosphatidic acid on growth, invasion and metastasis of ovarian cancer cells / 肿瘤

ABSTRACT

Objective: To investigate the effect of lysophosphatidic acid (LPA) on growth, invasion and metastasis of ovarian cancer cell lines SKOV3, 3AO and CAOV3. Methods: The ovarian cancer cell line 3AO, SKOV3 and CAOV3 were cultured and incubated with LPA at different concentrations for 2, 4, 6, 16 and 24 h. The control group was incubated with PBS instead of LPA. The effect of LPA on cell proliferation was detected by MTT assay. The invasion ability of ovarian cancer cells was evaluated by the Boyden chamber invasion test in vitro. The secretion of cytokines in the supernatant of cultured CAOV3 was measured by ELISA after being stimulated by LPA. Results: LPA interference greatly enhanced cell growth in a concentration-dependent manner. Boyden chamber invasion test showed that the invasion indices of 3AO and CAOV3 test groups were all higher than those of corresponding control groups, respectively. The difference was significant between different concentration groups. But the difference was not significant between the 3AO and CAOV3 cell lines. The expression of cytokines in supernatant fluid of CAOV3 cells had corresponding changes after LPA interference at different concentrations. Conclusion: LPA interference significantly enhances the growth of ovarian cancer cells in vitro. The invasion indices and the levels of migration-related cytokines have corresponding changes after LPA interference at different concentrations. It indicates that LPA might have the biologic significance in invasion and metastasis of epithelial ovarian cancer.