Effect of macrophages with different activated phenotypes on tumor growth, lymphangiogenesis and lymph node metastasis in mice bearing Lewis lung carcinoma / 肿瘤

ABSTRACT

Objective: To explore the effect of macrophages with different activated phenotypes on tumor growth, lymphangiogenesis and lymp node metastasis in mice bearing Lewis lung carcinoma (LLC). Methods: The xenografted models were established via subcutaneous injection of LLC cells and activated macrophages into mice. They were divided into 4 groups blank control group, classically activated macrophages (caMphi) group, alternatively activated macrophages (aaMphi) group and RAW264. 7 group with 10 mice in each group. The growth of transplanted tumors was examined dynamically and then the mice were sacrificed on d 28. The transplanted tumors were resected and the lymph nodes and distant metastasis were observed by HE staining. The LYVE-1+ lymphatic vessel densities (LVD) were detected by immunohistochemistry. Furthermore, other 40 mice were randomly divided into the four groups and the survival rates were recorded. Results: Compared with the blank control group, the aaMphi and RAW264. 7 xenografted tumors grew faster and had more lymph nodes and lung metastasis (P 0.05). Conclusion: aaMphi can promote the growth, lymphangiogenesis, lymph nodes and lung metastasis in transplanted LLC tumors, and reduce the survival rate of tumor-bearing mice. In the tumorigenesis of transplanted LLC tumors, RAW264. 7 cells may toward aaMphi.