The mechanism underlying the inhibitory effect of spleen tyrosine kinase on the invasion and migration of human breast cancer cells / 肿瘤

ABSTRACT

Objective: To investigate the effect of spleen tyrosine kinase (Syk) on invasion and migration in human breast cancer cells and analyze its action mechanism. Methods: Full-length cDNA of Syk was transfected into Syk-negative human breast cancer cells MDA-MB-231 via liposome mediation. The invasion and migration ability of MDA-MB-231 cells was detected by Transwell ECM method. The expressions of invasion and migration-related molecule vasculars, endothelial growth factor (VEGF) and matrix metalloproteinaseses-9 (MMP-9), were detected by flow cytometry (FCM) and enzyme-linked immunosorbant assy (ELISA) methods. Results: The number of membrane-passing cells every 5 high power fields was significantly decreased in Syk-transfected MDA-MB-231 cells compared with those transfected with blank vector or without transfection (P < 0.05). The expressions of VEGF and MMP-9 proteins were obviously reduced (P < 0.05). Conclusion: Syk cDNA inhibited the invasion and migration of tumor cells by down-regulating the expressions of VEGF and MMP-9.