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Effect of NF-kB antisense oligonucleotides on tumorigenesis and angiogenesis of gastric cancer cells in nude mice / 肿瘤
Tumor ; (12): 558-562, 2008.
Article in Chinese | WPRIM | ID: wpr-849331
ABSTRACT

Objective:

To investigate the effects of transfection of antisense oligodeoxynucleotide (ASODN) of nuclear transcription factor κB (NF-κB) on tumorigenesis and angiogenesis of human gastric cancer cell line SGC7901 in nude mice.

Methods:

ASODN targeted P65 promoter region of NF-κB was designed, synthesized, and transfected into gastric cancer cell line SGC-7901. Fluorescent staining was used to observe transfection efficacy. The growth-inhibiting rate of cells was measured by MTT assay in vitro. The subcutaneous xenograft model of human gastric cancer cells was established in nude mice and the tumor growth curve was observed. The microvessel density (MVD) was detected by HE staining, and the apoptotic index (AI) was quantitatively determined by terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate fluorescence nick end labeling (TUNEL).

Results:

The cell proliferation was significantly inhibited in P65 ASODN-transfected group in vitro (P < 0.05). In vivo tumor formation test showed that the tumor volume in nude mice in ASODN group was obviously smaller than that of other groups (P < 0.05) and the AI was significantly higher than that of other groups (P < 0.001). The MVD in ASODN group was markedly lower than that of other groups (P < 0.01).

Conclusion:

Transfection of P65 ASODN significantly inhibited the proliferation of SGC-7901 cells in vitro. The mechanism may be related to induction of cell apoptosis and inhibition of angiogenesis caused by P65 ASODN.

Full text: Available Index: WPRIM (Western Pacific) Type of study: Prognostic study Language: Chinese Journal: Tumor Year: 2008 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Type of study: Prognostic study Language: Chinese Journal: Tumor Year: 2008 Type: Article