The action-enhancing and toxicity-reducing effects of a new Coriolus versicolor polysaccharide peptide with cyclo-phosphamide on S180 sarcoma xenografted mice / 肿瘤

ABSTRACT

Objective: To investigate the effects of new Coriolus versicolor polysaccharide peptide (PSP) in enhancing the action and reducing the toxicity induced by cyclophosphamide (CTX) in mice bearing S180 sarcoma, analyze the related immunological mechanisms, and compare the effect of PSP2 (new polysaccharide peptide) with that of PSP1 (old polysaccharide peptide). Methods: S180 sarcoma xenografted mice models were established. The model mice were divided randomly into 8 groups, including model control group, CTX group, CTX plus PSP2 (200, 400, and 800 mg/kg) groups, CTX plus PSP1 400 mg/kg group, PSP2 400 mg/kg group, PSP1 400 mg/kg group. The normal blood cells and the nucleated marrow cells were counted. The survival time, the inhibitory rate of tumor growth, the index of thymus (thymus gland weight/body weight), spleen index (spleen weight/body weight) were measured. The subtypes of T lymphocytes in spleen were detected by flow cytometry. Results: PSP2 800 mg/kg combined with CTX prolonged the life span of the S180 sarcoma xenografted mice (P < 0.05). PSP2 and PSP1 alone obviously inhibited the growth of sarcoma, respectively (P < 0.05). PSP2 and PSP1 remarkably enhanced the ability of CTX to inhibit the growth of sarcoma as well (P < 0.05 or 0.01). PSP2 and PSP1 attenuated the immunological inhibition and marrow inhibition caused by CTX. PSP2 and PSP1 alone elevated the ratio of CD3+ CD4+/CD3+ CD8+ in mice bearing S180 sarcoma. The ratio tended to increase after combined treatment (PSP2 + CTX or PSP1 + CTX). Especially it increased remarkably after PSP2 400 mg/ kg combined with CTX (P < 0.05). Conclusion: PSP2 and PSP1 inhibited tumor growth, increased the action and reduced the toxicity of CTX. The mechanisms were related with enhancing the ratio of CD3+ CD4+/CD3+ CD8+ of spleen cells. PSP2 is effective than PSP1 in inhibiting tumor growth, enhancing the acion of CTX, and regulating the immunological function of nude mice.