Antisense oligodeoxynucleotides of Livin mRNA induced the apoptosis in human lung adenocarcinoma A549 cells / 肿瘤

ABSTRACT

Objective: To investigate the effect of antisense oligodeoxynucleotide (ASODN) of Livin mRNA on the proliferation and apoptosis of human lung adenocarcinorna A549 cells. Methods: Livin ASODN was transfected into A549 cells mediated by cationic liposome. The proliferation rates of A549 cells were assessed by MTT method. The transcription of Livin mRNA was detected by RT-PCR. The Livin protein expression was determined by immunohistochemistry and confocal laser scanning microscopy before and after transfection. The apoptotic ratios of A549 cells were examined by acridine orange/ethidium bromide(AO/EB) fluorescent staining. Results: It was observed that A549 cells expressed both Livin α and Livin β simultaneously, and the distribution of Livin protein could be observed in both cytoplasm and nuclei. The proliferation of A549 cells was inhibited significantly by Livin ASODN in a dose-dependent manner (P<0.01). After transfection of Lip-ASODN at a final concentration of 400 nmol/L, the expressions of Uvin were inhibited at mRNA and protein levels and the apoptotic ratio reached (31.25 ± 5.75)%. The difference was significant compared with control group [ (3.23% ± 1.98)%, P<0.01]. Conclusion: Livin ASODN significantly down-regulates the expression of Livin mRNA and effectively inhibites the proliferation and induces the apoptosis of A549 cells. Therefore Livin gene may become a new target for gene therapy for lung cancer.