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Silencing PI3K by siRNA activated FoxO3a gene and inhibited the growth of xenografted breast carcinoma in nude mice / 肿瘤
Tumor ; (12): 310-312, 2008.
Article in Chinese | WPRIM | ID: wpr-849388
ABSTRACT

Objective:

To investigate the effect of silencing PI3K by small interfering RNA (siRNA) on the expression of FOXO3a in human breast cancer MCF-7 cells and the growth of xenografted breast carcinoma in nude mice.

Methods:

Plasmids of siRNA for PI3K gene (Psilencer1.0-U6-PI3K) were constructed and xenografted tumor model was established in nude mice by subcutaneous inoculation of MCF-7 cells. Twenty-seven nude mice with xenografted tumor were divided randomly into 3 groups. Group 1 was intratumorally administered by Psilencer1.0-U6-PI3K plasmid. Group 2 and group 3 were intratumorally given Psilencer10-U6 plasmid and physiological saline (group 1), respectively. The tumor volume and survival time of nude mice were recorded. The expressions of PI3K and FOXO3a was measured by Western blotting.

Results:

The tumor size was significantly decreased and the average survival time was significantly enlongated in Psilencer1.0-U6-PI3K-treated group (P <0.01). Western blotting analysis showed that the protein expression of PI3K in xenografted tumor was markedly decreased and FOXO3a was significantly elevated compared with control groups (P <0.05).

Conclusion:

Psilencer1.0-U6-PI3K markedly silenced the expression of PI3K gene, up-regulated the expression of tumor suppressor gene FOXO3a, inhibited the growth of xenografted tumor, finally prolonged the survival time of nude mice bearing human breast carcinoma.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Tumor Year: 2008 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Tumor Year: 2008 Type: Article