Effects of inhibition of HER 2 expression by Newcastle disease virus on the proliferation of human gastric adenocarcinoma cell line BGC-823 / 肿瘤
Tumor
;
(12): 944-947, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-849454
ABSTRACT
Objective:
To investigate the effects of selective inhibition of HER 2 expression by Newcastle disease virus to the proliferation of human gastric adenocarcinoma cell line BGC-823.Methods:
The expression of HER 2 mRNA and protein in Newcastle disease virus-infected gastric adenocarcinoma cells was determined by RT-PCR and Western blotting. The inhibitory effect of Newcastle disease virus on proliferation of BGC-823 cells were assessed by MTT assay. Apoptotic rate of BGC-823 cells was measured by flow cytometry (FCM).Results:
The infection of BGC-823 cells with Newcastle disease virus successfully knocked down the expression of HER 2 at mRNA and protein levels. Down-regulation of HER 2 gene inhibited the proliferation and increased the apoptotic rate of BGC-823 cells compared with the control group (P < 0.01).Conclusions:
Infection with Newcastle disease virus efficiently killed BGC-823 cells and induced apoptosis. The mechanism may be due to the inhibition of HER 2 gene expression in BGC-823 cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Tumor
Year:
2007
Type:
Article
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