Studies on the mechanism underlying androgen-independent prostate cancer invasion and metastasis promoted by vimentin / 肿瘤

ABSTRACT

Objective: To study the molecular mechanism underlying androgen-independent prostate cancer invasion and metastasis promoted by vimentin. Methods: With specific amplified primer of vimentin, full-length vimentin and full-length antisense-vimentin cDNA fragments were synthesized by reverse transcriptase polymerase chain reaction ( RT-PCR). RT-PCR products were cloned into plasmid pcDNA3.1 (+) and pcDNA3.1 (-). The recombinant vimentin plasmids were transfected into androgen-independent prostate cancer PC-3M-2B4 and PC-3M-1E8 cells. The positive clones were screened by G418. The transwell chamber migration assay was used to study cell invasion. Western blotting was used to determine specific protein expression in the cells. Results: Vimentin promoted androgen-independent prostate cancer cell invasion and metastasis by regulating the E-cadherin/β-catenin complex via C-src kinase. Conclusion: Vimentin could be used as an important prognostic factor for androgen-independent prostate cancer cell invasion and metastasis.