Association of the MDM2 polymorphisms with susceptibility of esophageal squamous cell carcinoma and that of gastric cardiac adenocarcinoma / 肿瘤

ABSTRACT

Objective: To investigate the correlation between the SNP309 and Del1518 polymorphisms in the promoter region of murine double minute 2 (MDM2) and genetic susceptibilities of esophageal squamous cell carcinoma (ESCC) and gastric cardiac adenocarcinoma (GCA). Methods: Genotypes of the MDM2 polymorphisms of 563 cancer patients (351 ESCC and 212 GCA) and 642 healthy controls were detected by a primer-introduced restriction analysis-polymerase chain reaction (PIRA-PCR). The combined effects of MDM2 polymorphisms were analyzed by EH and 2LD softwares. Results: Distributions of the MDM2 genotypes in both ESCC patients and healthy controls were not significantly different (P > 0.05), however, a significant different distribution between GCA patients and healthy controls was observed (P < 0.05). The MDM2 haplotype distributions were not significantly different between ESCC patients and healthy controls (P = 0.198). MDM2 haplotype distribution in the GCA patients was also significantly different from that in the healthy controls (P 0. 000). Compared with SNP309G/Del1518 + haplotype, SNP309T/Del1518 - haplotype significantly decreased the risk of developing GCA (adjusted OR = 0.51, 95% CI = 0.38-0.70). Conclusions: MDM2 polymorphisms may not be associated with susceptibility to ESCC. Individuals with MDM2 SNP309T allelotype (G/T and T/T genotype) and with Del1518 - allelotype (+/- and -/- genotype) have significantly lower risk of developing GCA, respectively. Individuals T/Del1518 - haplotype have significantly lower risk of developing GCA.