The effect of captopril on the levels of transforming growth factor β1 and tumor necrosis factor α in mice after lung radiation injury / 肿瘤

ABSTRACT

Objective: To investigate the effect of angiotensin-converting enzyme inhibitors (ACEI), captopril (Cap), on the serum levels of transforming growth factor β1 (TGF-β1) and tumor necrosis factor α (TNF-α) in mice after lung irradiation injury. Methods: Male C57/BL mice were divided into three groups control group, right lung single irradiation group (10 Gy), and right lung single irradiation (10 Gy) plus Cap administration group. The blood samples were collected at 36 h, 7 d, 15 d, and 30 d after irradiation. The levels of TGF-β1 and TNF-α were evaluated with ELISA method. The right lungs were isolated and stained with HE for pathological examination at the same time points. Results: The average serum levels of TGF-β1 and TNF-α in single 10-Gy irradiation group were significantly increased at different time points compared with the control group. But they were significantly decreased at different time points in 10-Gy radiation plus Cap group compared with the control group. The results of analysis of variance showed that there was a significant difference among the factor of time, the factor of treatment, and the factor of interaction (P < 0.05). Pathological examination showed that acute inflammation was aggravated in single 10-Gy irradiation group. But there was no significant difference in lung injury score between 10-Gy irradiation plus Cap group and control group. Conclusions: Cap reduces the serum levels of TGF-β1 and TNF-α and prevents lung against radiation-induced injury.