Effect of c-myc antisense oligodeoxynucleotide on proliferation and apoptosis of colon cancer SW480 cells with FHIT gene re-expression / 肿瘤

ABSTRACT

Objective: To study the effect of the c-myc antisense oligodeoxynucleotide (ASODN) on the proliferation and apoptosis of colon cancer cells with FHIT gene expression. Methods: PRC/CMV vector expressing FHIT gene and empty vector were transfected into human colon cancer SW480 cells by liposome mediation, respectively. Then, c-myc ASODN was transfected into SW480 cells. The FHIT and c-myc proteins were detected by western blotting. The proliferation of SW480 cells was assessed by MTT assay. The apoptosis was determined by AO/EB staining and flow cytometry analysis. Results: Marked expression of FHIT protein was detected in SW480 cells after transfection with PRC/CMV vector. But it was not observed in SW480 cells after transfection with empty vector. The expression of c-myc protein was significantly inhibited in SW480 cells after transfection with c-myc ASODN ( P < 0.01 ). ASODN of c-myc inhibited the proliferation and induced the apoptosis of SW480 cells. However the growth-inhibiting and apoptosis-inducing effect of c-myc ASODN were stronger on FHIT + SW480 cells compared with FHIT - SW480 cells (P < 0.05). Conclusions: The expression of tumor suppressor gene FHIT and down-regulation of oncogene c-myc have stronger anti-tumor efficiency and provides the basis for multiple gene interference therapy.