Heat stroke induces oncosis rather than apoptosis in skeletal muscle cells / 解放军医学杂志

ABSTRACT

Objective To investigate the oncosis of skeletal muscle cells during heatstroke, and the mechanism severe heatstroke associated with rhabdomyolysis. Methods The human skeletal muscle cells (HSKMC) were divided into control group and heat shock group. The control group was incubated at 37 ℃, 5% CO2 cell incubator, while the heat shock group was exposed at 43 ℃, 5% CO2 cell incubator for 2 hours, followed by incubating at 37 ℃, 5% CO2 cell incubator. At incubation point of 0, 6, 12, and 24 hours, the CCK-8 method was used to detect cell viability; the LDH method was used to detect cytotoxicity; the transmission electron microscope was used to detect cell ultrastructure changes; Annexin -FITC/PI double staining method was used to detect double-positive cell rate; Western blotting method was used to detect porimin and caspase-3 protein expression. Results Compared with the control group, HSKMC cell viability decreased with cytotoxicity increased at 0 h after rewarming in a time-dependent manner (P0.05). Conclusion Heat stroke-induced oncosis rather than apoptosis of skeletal muscle cells.