Clinical diagnostic value of plasma exosomes S100P protein in patients with heatstroke / 解放军医学杂志

ABSTRACT

Objective: To investigate the plasma exosome S100P levels in patients with heatstroke and evaluate its clinical diagnostic value. Methods: Thirty heatstroke patients were selected from June 2016 to June 2018 in the General Hospital of Southern Theatre Command of PLA, and 15 healthy people in the Physical Examination Center of our hospital were selected as control. The venous blood was collected, followed by plasma exosomes extraction using polyethylene glycol precipitation. The plasma exosome S100P was quantified by ELISA. The correlation analysis between plasma exosomal S100P, clinical indicators, and disease severity was performed. The receiver operating characteristic (ROC) curve was analyzed to evaluate the efficacy of plasma exosome S100P in diagnosing heatstroke. Results: The level of plasma exosomes S100P in patients with heatstroke significantly increased [healthy people vs. heatstroke patients (195.89 ± 131.97) pg/ml vs. (1163.62 ± 747.15) pg/ml, P<0.001], and the level of plasma exosome S100P was significantly correlated with the severity of heatstroke patients [Mild heatstroke patients vs. severe heatstroke patients (553.63 ± 311.85) pg/ml vs. (1570.28 ± 672.03) pg/ml, P<0.001]. The correlation coefficients r of plasma exosome S100P with APACHE II score, SOFA score and MODS score were 0.888, 0.901, and 0.887, respectively (P<0.001). The area under the ROC curve for the diagnosis of heatstroke by plasma exosomal S100P was 0.931 (95%CI 0.857-1.000, P<0.001). The area under the ROC curve of plasma exosomal S100P for assessing the severity of heatstroke was 0.956 (95%CI 0.890-1.000, P<0.001). Conclusion: The level of plasma exosomal S100P correlates with severity of heatstroke and can be used as a potential marker for diagnosis and prognosis of heatstroke.