Expression and significance of mir-345-5p in serum of patients with hyperlipidemic acute pancreatitis / 解放军医学杂志

ABSTRACT

Objective To investigate the expression of miR-345-5p in peripheral blood of patients with hyperlipidemic acute pancreatitis (HAP) and its clinical significance. Methods From December 2017 to September 2018, 40 patients with HAP (group A) and 75 patients with non-HAP (group B) admitted to the general surgery center of the General Hospital of Western Theater Command were enrolled. 25 patiens with hyperlipidemic non-acute pancreatitis patients (group C) and 30 healthy volunteers (group D) were selected as the control group. Group A was subdivided into H-MAP group and H-SAP group. All the patients were examined for serum triacylglycerol and amylase biochemical levels, and scored on the APACHE II. The expression level of miR-345-5p in serum was detected by real-time fluorescent quantitative PCR. The relationship between miR-345-5p expression level and clinical indicators of HAP was analyzed and the role of miR-345-5p in predicting the severity of acute pancreatitis evaluated. Results The expression level of miR-345-5p in serum of group A (2.28±0.23) was significantly higher than that of groups B (0.35±0.21), C (0.29±0.23) and D (0.25±0.33). The difference was statistically significant (P0.05). The ROC curve analysis showed that the area under the curve of miR-345-5p detection H-SAP was 0.785(0.634~0.935). The expression of miR-345-5p in the HAP severe group (2.95±0.46) was significantly higher than that in the mild HAP group (0.69±0.23), the difference was statistically significant (P0.05). Conclusion The serum miR-345-5p is significantly up-regulated in HAP, and it increases with the severity of the disease, which may provide a new way for the clinical diagnosis and severity assessment of HAP.