Effects of down-regulation of TRIM33 on proliferation and invasion of human gastric cancer cell BGC-823 / 解放军医学杂志

ABSTRACT

Objective To explore the potential function of TRIM33 in the progression of gastric cancer using human gastric cancer BGC-823 cells with down-regulated TRIM33. Methods Recombinant plasmid si-TRIM33 and control vector were transfected into BC-823 cells using Liposome 2000. The protein level of TRIM33 was detected by Western blotting. CCK-8 and EdU assay were used to evaluate cell proliferation. Plate cloning assay was employed to exam the clone formation rate and the scratch test was used to assess cell invasion ability. Results Comparing to cells that transfected with the control vector (control group), cells transfected with si-TRIM33 vectors (si-TRIM33 group) showed dramatically decreased levels of TRIM33 expression (P<0.01). Cells transfected with the control vectors showed the OD value of 0.48±0.06 and 0.51±0.11 at 24h and 48h, respectively; while cells transfected with si-TRIM33 vectors showed the OD values of 0.75±0.02 and 1.16±0.10 at 24h and 48h, respectively, using CCK-8 test. Therefore, knocking down TRIM3 in BGC-823 cells promotes cell proliferation (P<0.01). This is further confirmed using EdU assay. The cell proliferation rates of cells transfected with control vector and cells with the si-TRIM33 vectors were 14.43±1.79 and 42.92±5.05, respectively (P<0.01). In the plate cloning formation experiment, the number of spheres in the control group and the si-TRIM33 group were 675.00±141.52 and 1233.00±242.62, respectively. This showed that the down-regulation of TRIM33 enhances the colony formation rate (P<0.05). In the invasion experiment, scratch healing rates of the control group and the si-TRIM33 group at 12h were 0.105±0.018 and 0.169±0.032, respectively. The healing rates at 24h in the control group and the si-TRIM33 group were 0.167±0.016 and 0.258±0.031, respectively. Together, decreased TRIM33 enhances cell invasion (P<0.05). Conclusion Down-regulation of TRIM33 expression promotes proliferation and invasion of human gastric cancer BGC-823 cells.