Association between CMTM5 gene rs723840 single nucleotide polymorphism and coronary artery disease / 解放军医学杂志

ABSTRACT

Objective To elucidate the correlation between the single nucleotide polymorphism ofCKLF-like MARVEL transmembrane domain containing member 5 (CMTM5) gene rs723840 and the occurrence of coronary artery disease (CAD). Methods The present study is a case-control study. A total of 1110 hospitalized patients in Shijitan Hospital were enrolled in this study. Patients were divided into CAD group (n=560) and control group (n=550). CAD were diagnosed by coronary angiography, which was defined as at least one blood vessel diameter stenosis ≥50% according to the result of coronary angiography. Genotypes were determined by polymerase chain reaction (PCR) and using sequencing analysis to detect rs723840 of CMTM5 gene. The proportions of genotype and allele of CMTM5 gene were analyzed. Results The genotype frequencies in rs723840 C>T of CMTM5 gene conformed well to the Hardy-Weinberg equilibrium in both CAD group and control group. Between the two groups, the genotypes frequency (CC, CT and TT) in CAD and control groups were 53.9%, 40.9%, 5.2% and 69.3%, 28.7%, 2.0%, respectively (P<0.001). T allele frequency was significantly higher than that in C allele frequency (25.6% vs. 16.4%, OR=1.566, 95%CI 1.325-1.850, P<0.001). After adjusted for the risk factors of age, gender, BMI, smoking, hypertension, diabetes and hyperlipidemia, logistic regression analysis results indicated that CMTM5 was the susceptibility factors of CAD, which showed significant correlation with CAD. Conclusions A significant correlation was found between CMTM5 gene rs723840 polymorphism and the occurrence of CAD, T allele carriers are closely related to the occurrence of CAD.