Effect of Paeonia lactiflora formula granule against the damage of LO2 cells induced by carbon tetrachloride / 解放军医学杂志

ABSTRACT

Objective To investigate the effect of Paeonia lactiflora formula granule (PLFG) against the damage of LO2 cells induced by carbon tetrachloride (CCl4) and explore its mechanism. Methods LO2 cells were divided into control group, CCl4 model group, PLFG (1, 5, 10mg/L) groups and Vit E (50mmol/L) group. LO2 cells of PLFG groups and Vit E group were pretreated by drugs for 24 hours, then except control group, other groups were treated by 10mmol/L CCl4 for 6 hours, which induced hepatic cell damage, the alanine aminotransferase (ALT) and aspartate transaminase (AST) content of the cell culture supernatant were measured, the survival rates of LO2 cells were analyzed by MTT method. LO2 cells were divided into control group, CCl4 model group, PLFG (10mg/L) group and Vit E (50mmol/L) group, cell loss, changes in nuclear size and morphology, DNA content, mitochondrial membrane potential (MMP), cell permeability changes, and cytochrome C release were measured simultaneously by high content analysis (HCA). Results Compared with CCl4 model group, PLFG and Vit E pretreatment could obviously decrease the level of ALT and AST (P<0.05 or P<0.01), especially in the 10mg/L PLFG group and Vit E group. PLFG (1, 5, 10mg/L) and Vit E could also significantly weaken the decrease of LO2 cell viability, which were induced by CCl4 treatment, the results showed in dose-dependent to some extents (P<0.05 or P<0.01). Meanwhile, treatment with 10mg/L PLFG and Vit E could significantly increase the level of MMP (P<0.01), prevent cytochrome C release (P<0.01), decrease the membrane permeability (P<0.01), increase nuclear size (P<0.01), decrease total nuclear intensity (P<0.01) and increase the cell count (P<0.05). Conclusion PLFG may act an obvious protective effect against the liver injury induced by CCl4, and it might be due to protecting the integrity of mitochondria membrane, decreasing cell membrane permeability and inhibiting the apoptosis of LO2 cells.