Effect of bicyclol on liver tissue proliferation in mice with tetracycline-induced fatty liver / 解放军医学杂志

ABSTRACT

Objective To investigate the effect of bicyclol on liver tissue proliferation in mice with tetracycline-induced fatty liver. Methods Fifty ICR mice were randomly assigned into 5 groups (10 each) normal group (control), tetracycline 6h group, bicyclol 6h group, tetracycline 24h group and bicyclol 24h group. Mice in the two bicyclol groups received bicyclol (300mg/ kg) by gavage for 3 times in 12h interval, meanwhile, those in the other groups received the equal amount of forming agents. Tetracycline (200mg/kg) was then injected intraperitoneally to the mice in bicyclol groups and tetracycline groups 1h after the last dose of bicyclol, and mice in control group received equal amount and pH of saline. The liver issues and blood samples from eyes were collected 6h and 24h after tetracycline injection for detection of the corresponding indicators. RT-PCR was performed to detect the gene expression of proliferation index (PCNA, cyclin D1 and c-myc), Western blotting was performed to detect the expression of Cyclin D1 in liver tissue, and immunohistochemistry was employed to detect the expression of PCNA and c-myc proteins. Results The expression level of PCNA gene 6h after tetracycline injection in mice of tetracycline group was 43% of that in control group, meanwhile the expression of protein also reduced obviously. Bicyclol remarkably inhibited the decrease of PCNA gene and protein expressions at early time (6h) of fatty liver. In addition, the expression of cyclin D1 gene in tetracycline 24h group was 59%, and the expression of c-myc was 5.7 folds of that in control group. Bicyclol remarkably up-regulated the gene expressions of cyclin D1 and c-myc 6h after tetracycline injection, and up-regulated the protein expression of cyclin D1 24h after tetracycline injection. Conclusion Bicyclol can enhance hepatocyte proliferation of mice with tetracycline-induced fatty liver by up-regulation of PCNA, cyclin D1 and c-myc expression.