Value of serum soluble CD30 as a biomarker for radiation exposure / 解放军医学杂志

ABSTRACT

Objective To assess the feasibility of serum soluble CD30 (sCD30) as a biomarker for radiation exposure. Methods Three hundred male C57BL/6 mice were prepared and randomly divided into 4 radiation groups (60 each) and control (n=60). Mice in radiation groups were treated with 60Co total-body irradiation in the dose of 1, 3, 5 or 7 Gy, and the dose rate of 60Co ray at one-time whole-body irradiation was 0.78 Gy/min. 24, 48, or 72h after radiation, the serum samples were obtained (20 mice in each group at each time point). The serum sCD30 concentrations were analyzed with custom-made antibody array, which was purchased from RayBiotech. To further analyze the relationships of serum sCD30 concentration and radiation dose, regression analysis was used. Results The baseline level of sCD30 in serum of non-exposed animals was 121.2±25.61pg/ml. Moreover, serum sCD30 concentration was inversely related to the radiation dose during 72h after exposure. Regression analysis showed an obvious dose-effect relationship between serum sCD30 concentration and radiation dose (24h after radiation, R2=0.8715; 48h after radiation, R2=0.9024; 72h after radiation, R2=0.9913). Using these equations, radiation exposure can be easily estimated by the serum sCD30 value. Conclusions The baseline level of serum sCD30 remains steady without radiation. Further regression analysis uncovers a strong correlation between serum sCD30 concentration and radiation dose, and serum sCD30 may be used to estimate radiation exposure as a biomarker.