Study on radioprotection effects of clinoptilolite on sub-acute radiation-injured mice / 解放军医学杂志

ABSTRACT

Objective To study the protection effect of clinoptilolite (Cp) against radiation injury. Methods Forty-eight male BALB/c mice were randomly divided into 6 groups, namely normal control group (distilled water+sham irradiation) radiation control group (distilled water + irradiation), 300mg/kg 523 group (200mg/kg nilestriol 24h before irradiation and 100mg/ kg nilestriol 4h after irradiation), 56mg/kg Cp group (56mg/kg Cp + irradiation), 167mg/kg Cp group (167mg/kg Cp + irradiation) and 500mg/kg Cp group (500mg/kg Cp+irradiation). Seven days after the administration of the drug, all the mice but those from the normal control group were irradiated with γ-ray irradiation of137Cs in the dose of 4.0Gy, at the rate of 0.75Gy/min. All the mice were given the drug for 14 days after irradiation. RBC, WBC and PLT counts in peripheral blood, superoxide dismutases (SOD) activity, malondialdehyde (MDA) level, glutathione peroxidase (GSH-Px) level in blood serum, the content of deoxyribonucleic acid (DNA) and hematopoietic stem cells in bone marrow were determined. Results Compared with radiation control group, on 10th day after irradiation, RBC counts in peripheral blood of 56mg/kg Cp group and 167mg/kg Cp group were significantly higher (P<0.05, P<0.0l), and WBC counts in peripheral blood of S6mg/kg Cp group and 500mg/kg Cp group were significantly higher (P<0.0l). On 14th day after irradiation, compared with radiation control group, the SOD activities in blood serum of three Cp groups were elevated (P<0.05, P<0.01) the GSH-Px levels were elevated in blood serum of 167mg/kg Cp group and 500mg/kg Cp.