Effect of P I CP/P III NP on assessing cardiac diastolic dysfunction and collagen remodeling / 解放军医学杂志

ABSTRACT

Objective To explore the changes of serum procollagen I carboxyl peptide (P I CP)/procollagen III aminoterminal peptide (P III NP) levels in cardiac diastolic dysfunction rabbit model, and the relationship with cardiac diastolic dysfunction and collagen remodeling. Methods Sixty rabbits were randomly divided into control group (control), myocardial infarction group (MI) and left ventricular hypertrophy group (LVH)(20 each). E/e' ratio was detected by flow Doppler and tissue Doppler imaging, and ELISA was performed to detect the P I CP/P III NP level at the 2nd, 4th, 8th and 12th week. The collagen volume fraction (CVF) and I/III collagen ratio were detected with polarized light through picric-acid Sirius red staining at the 12th week. Results The P I CP and P III NP levels increased in MI group and LVH group than in control group with statistically significant difference (P<0.05), and the P I CP and P III NP levels were also increased in MI group than in LVH group (P<0.05) in a time-dependent manner; The E/e' ratio increased in MI group and LVH group than in control group with statistically significant difference (P<0.05) in a time-dependent manner; The CVF increased in MI group and LVH group than in control group with statistically significant difference (P<0.05). The P I CP and P III NP levels were positively correlated with the E/e' ratio (P<0.05), and the P I CP and P III NP ratio was positively correlated with the I/III collagen ratio (P<0.05). Conclusion The P I CP and P III NP levels can be used to evaluate the changes of cardiac diastolic function and myocardial collagen remodeling in different pathological conditions.