Effects of positive acceleration exposure on intestinal mucosal barrier and sIgA level in rats / 解放军医学杂志

ABSTRACT

Objective To explore the effect of positive acceleration (+Gz) on immune barrier of intestinal mucosa in rats. Methods Thirty two male SD rats were randomly divided into 4 groups (8 each) Group A (control group), Group B (+5Gz group), Group C (+10Gz group) and Group D (repeated exposure group). The animal centrifuge was used to simulate the exposure of acceleration. Group A was no disposed. +5Gz group and +10Gz group were subjected to centrifugal force of +5Gz and +10Gz respectively for 5min; repeated exposure group was continuously exposed to 1.5min under +5Gz value, 2min under +10Gz value and 1.5min under +5Gz. All groups were exposed to the respective acceleration once daily for 5 days. The damage of intestinal mucosa was observed by light microscopy after the experiment was finished, and the content of sIgA in intestinal mucosa was detected by ELISA. Results Except for group A, intestinal mucosal injury was observed in the other three groups. Group D was shown as the most serious one, followed by group C and group B. Compared with group A, the level of sIgA was significantly lower in other three groups (P<0.05). The level of sIgA in group C was significantly lower than that in group B (P<0.05) and higher than that in group D (P<0.05). Conclusion +Gz exposure can result in intestinal injury and weaken the function of immune barrier of intestinal mucosa in rats.