Effects of eIF6 on the expression of pro-inflammatory mediators derived from M2 macrophages / 解放军医学杂志
Medical Journal of Chinese People's Liberation Army
;
(12): 104-108, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-850151
ABSTRACT
ObjectiveTo explore the effects of eukaryotic translation initiation factor 6 (eIF6) on the expression of fibrotic cy tokines and metabolic enzymes derived from M2 macrophages. MethodseIF6 wild type mice (eIF6+/+) and eIF6 knock out mice (eIF6+/-) were used. Macrophages were collected from peritoneal lavage fluid, and they were stimulated with IL-4 for enrichment of M2 macrophages. The differential gene expressions of eIF6+/+ and eIF6+/- M2 macrophages were studied with gene chip. The differential expression of genes was further confirmed with both RT-PCR and ELISA. ResultsThe gene chip showed that the expressions of VEGF and TIMP-2 were down-regulated in eIF6+/+ macrophages compared with those in eIF6+/- M2 macrophages, while the expression of MMP-2 was up-regulated in eIF6+/+ M2 macrophages (P<0.05). The result of RT-PCR and ELISA had confirmed that the RNA and protein expressions of VEGF and TIMP-2 were decreased in eIF6+/+ macrophages compared with those in eIF6+/- M2 macrophages, while the RNA and protein expressions of MMP-2 were up-regulated in eIF6+/+ M2 macrophages (P<0.05). ConclusioneIF6 not only inhibits the expression of VEGF, reduces angiogenesis and granulation tissue formation, but also enhances the extracellular matrix degradation and deposition by regulating the balance of MMP2/TIMP2, and thus attenuating the degree of fibrosis (scar formation).
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Medical Journal of Chinese People's Liberation Army
Year:
2015
Type:
Article
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