Association between the single nucleotide polymorphism of RASSF1A Ala133Ser and colorectal cancer in north Chinese Han population / 解放军医学杂志

ABSTRACT

Objective To evaluate the association between RASSF1A Ala133Ser single nucleotide polymorphism (SNP) and colorectal cancer (CRC) in patients of Han population in north China. Methods RASSF1A SNP was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis in 109 colon cancer (CC) patients, 67 rectal cancer (RC) patients and 189 age and sex matched healthy people (as controls). Unconditional logistic regression was used to analyze the gene-disease association and gene-sex interaction. Results The RASSF1A Ala133Ser SNP in CRC patients had no significant difference with that in normal control samples, but the interaction of genotype and sex existed in rectal cancer, Ala/Ser +Ser/Ser genotypes of RASSF1A gene increased the risk of rectal cancer in women (OR=3.78, 95%CI 1.31-10.89). Conclusion The RASSF1A 133Ser allele is associated with rectal cancer in women, but not colon cancer.