Effects of interceed on endometrial receptivity in rabbits with intrauterine adhesion / 解放军医学杂志

ABSTRACT

Objective To observe the effect of anti-adhesion membrane made of oxidized regenerated cellulose (Interceed) on endometrial receptivity in New Zealand white rabbits with intrauterine adhesion (IUA). Methods-Forty-eight female rabbits were randomly divided into 4 groups (12 each) normal control group (group A), Interceed control group (group B), untreated model group (group C), and Inte rceed therapy group (group D). Rabbits in group A received sham operation and underwent no modeling. The Interceed was placed into the normal rabbits' uteruses in group B. The IUA rabbit models were reproduced in group C and group D by both mechanical injury and infection. Rabbits in group C were not treated, and in group D Interceed was put into their uteruses 7 days after modeling. Four rabbits in each group were sacrificed on the 28th day, the uterine tissues were collected. The endometrial glands count was performed after HE staining, and the degree of endometrial fibrosis was assessed after Masson staining. For the remaining animals in each group, ovulation was induced by stimulating the vagina in estrus, and they were sacrificed and the uterine tissue was collected on the 7th day after pseudopregnancy. The expression of pinopodes in endometrium was observed by scanning electron microscopy, and that of integrin αvβ3 protein was determined by immunohistochemistry. Results -The expression of glands and the degree of fibrosis in endometrium was obviously improved in group D as compared with that of group C (P<0.05), but no significant difference was found among the groups A, B and D. In group A, and developed pinopodes were abundantly expressed in endometrial surface, and similar changes were found in group B. The expression of pinopodes in group C was scarce, and the development was not synchronized. More pinopodes, with irregular shape and synchronized development, were expressed in group D than in group C. The expression of integrin αvβ3 protein was significantly lower in group C than in group A and group D (P<0.05). Conclusion-Interceed may alleviate the endometrial pathological changes, increase the expression of integrin αVβ3 protein, ameliorate the expression and development of pinopodes, thus improve the endometrial receptivity.