Experimental study of effects of new bioactive materials containing type I collagen in repairing bone defect / 解放军医学杂志

ABSTRACT

Objective To evaluate the osteogenic potential and biodegradability of three kinds of new bioactive bone graft materials. Methods Eighteen healthy adult female New Zealand white rabbits were selected. A bone defect with a hole of 6 mm in diameter and 12 mm in depth in cancellous bone of bilateral femoral condyles was established by operative method, and 1.5-2.0ml bone marrow was harvested from the proximal tibia. Then the animals were randomly divided into three groups (n=6), and the bone defects were filled with autologous bone marrow mixed NovaBone Collagen Bioactive Glass Strips (group A), NovaBone Collagen Bioactive Glass Morsels (group B), or Orthovita Vitoss Foam Pack(group C),respectively. Specimens were collected at 6 and 12 weeks respectively after operation to evaluate the osteogenic potential and biodegradability through gross observation, X-ray radiograph and histological examination. Results Gross observation showed bony fusion of the defects with adjacent bone tissues 12 weeks after operation and the boundaries were difficult to be distinguished. Lane-Sandhu X-ray scores and histological observation showed that in terms of the osteogenic potential and biodegradability, group A was better than groups B and C(P<0.05), and group B was better than group C (P;0.05) 6 and 12 weeks after operation. Compared with the results as observed at 6 weeks after operation, the amounts of bone formation and material degradation were all increased 12 weeks after operation in all the 3 groups (P<0.01). Conclusions NovaBone Collagen Bioactive Glass Strips have favorable biocompatibility, stable osteogenic potential and biodegradability, and may be used for repairing bone defects.