Effects of tenghuanglin on injury to splenic lymphocyte induced by microwave radiation in rats / 解放军医学杂志

ABSTRACT

Objective To observe the effects of Tenghuanglin (THL) on injury to peripheral T and B lymphocytes induced by microwave radiation in rats, and explore the protective effects of THL against derangement of immunity in rat injury induced by microwave irradiation and its mechanism. Methods Eighty clean male SD rats were randomly divided into normal group (CON group), radiation group (RAD group), AduoLa Fuzhenglin (ADL) treatment group and THL treatment group, with 20 rats in each group. Before radiation, rats in ADL group and THL group were treated with ADL and THL respectively by gavage once per day for 7 days. Then, whole body of the rats was respectively exposed to 30 mW/cm2 microwave for 15 min. Rats in CON group were sham-radiated. The changes in splenic CD3+, CD4+, CD8+ T lymphocyte subsets and CD4SRA+ B lymphocyte subset were analyzed 7 and 14 days after radiation. Results Seven days after radiation, the splenic coefficient of RAD group was lower than that of CON group and THL group (P0.05). The CD4SRA+ B cell proportion of RAD group was lower than those of CON group and ADL group 7 days after radiation (P0.05). Conclusions The splenic T and B lymphocytes subsets decrease significantly at the early stage after microwave radiation in rats. Because of the rapid decrease in CD4+ T cell proportion, decreased CD4/CD8 ratio could lead to immune imbalance. Preventive treatment with THL could increase the T and B lymphocyte proportions and improve the CD4/CD8 ratio in rats after microwave radiation.