Heat shock protein 70-2 gene polymorphism and susceptibility to diabetic nephropathy / 解放军医学杂志

ABSTRACT

Objective To investigate the relationship between heat shock protein 70-2 (HSP70-2) gene polymorphism and diabetic nephropathy (DN) in patients with type 2 diabetes mellitus (T2DM). Methods Following the inclusion and exclusion criteria, a total of 677 subjects were enrolled in present study. They were either hospitalized or undergoing regular health check-up in the Department of Nephrology of PLA 152nd Hospital in Pingdingshan of Henan Province. Among them 226 patients were suffering rom diabetes mellitus and nephrosis (DN group), 221 patients with 2DM but without nephrosis (DN group), and 230 were normal controls (NC group). Data were collected by questionnaire, physical examination and laboratory examination, and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was applied to detect the single nucleotide polymorphisms of HSP 70-2(+1267/G). Results The G/G genotype frequency of HSP70-2 was significantly higher in DN group than in DN and NC group (χ2 =8.123, P<0.01; χ2 =11.651, P<0.01); the G allele frequency of HSP70-2 was significantly higher in DN group than in DN and NC group (χ2 =9.392, P<0.01, OR=1.782; χ2 =11.971, P<0.01, OR=2.153). The levels of MALB/Cr and BMI were significantly higher in G/G gene carriers than in A/A gene carriers (P<0.05). Conclusion The G/G genotype of HSP70-2(+1267) maybe related to susceptibility to DN and general obesity; G allele carrier may have increased risk of T2DM with DN.