Effect of liraglutide on serum AGEs and inflammatory factors in IGT-OLETF rats / 解放军医学杂志

ABSTRACT

Objectiven To investigate the effect of liraglutide on serum advanced glycosylated end products (AGEs), inflammatory factors and glycometabolism in OLETF rats with impaired glucose tolerance (IGT). Methods Thirty-two 12-weekold OLETF rats were randomly assigned into 4 groups (8 each) and received intraperitoneal injection of saline (OLETF-saline group) or liraglutide 50, 100 or 200μg/kg twice a day for 12 weeks (liraglutide intervention groups). Eight LETO rats served as the normal control group and received saline injection. Twelve weeks after the treatment, all the rats were examined for fasting and 2h postprandial plasma glucose after Oral Glucose Tolerance Test (OGTT), and serum AGEs and inflammatory factors were determined by enzyme linked immunosorbent assay (ELISA). Results Before liraglutide intervention, the levels of fasting plasma glucose (FPG), plasma glucose 2 hours after OGTT (2hPG), and fasting plasma insulin (FPI) were significantly higher in IGT-OLETF rats than in LETO rats. While 12 weeks after liraglutide intervention, the levels of FPG, 2hPG, FPI, AGEs and the inflammatory factors TNF-α, Hs-CRP and IL-6 declined obviously in IGT-OLETF rats compared with that in OLETF-saline rats (P<0.05). When the liraglutide intervention ended, 87.5% of the rats in OLETF-saline group developed diabetes, but no sign of diabetes was found in the rats of liraglutide intervention groups. Conclusion Liraglutide may effectively improve IGT and insulin-resistance, decrease the AGEs and inflammatory factors by inhibiting nonenzymatic glucosylation and inflammation to delay or prevent the development of diabetes in IGT-OLETF rats.