Quantitative comparison of lung pathological processing and its diagnostics significance / 解放军医学杂志

ABSTRACT

Objective To qualitatively and quantitatively compare the effect of tracheal inflation method, vascular perfusion method and combined trachea-vascular perfusion method on alveolar structure and cell antigen preservation. Methods Fifteen healthy Sprague Dawley rats were randomly divided into three groups (5 each) tracheal inflation group, vascular perfusion group and combined trachea-vascular perfusion group. After lungs were respectively fixed with the methods mentioned above, tissue blocks were paraffin embedded and sectioned. HE staining and immunofluorescence staining were then performed for qualitative observation and stereological analysis of the fixation effect. Results Qualitative study showed that the fixation effect of cytoplasm and nuclear antigen was better in vascular perfusion group and combined trachea-vascular perfusion group than in tracheal inflation group. Stereological analysis showed that the alveolar linear intercept in combined trachea-vascular perfusion group was longer than that in tracheal inflation and vascular perfusion group (P<0.05). The lung parenchyma volume density and alveolar septum thickness of combined trachea-vascular perfusion group were smallest among all the three groups, followed by tracheal inflation group and vascular perfusion group in turn (P<0.05). Conclusions The combined trachea-vascular perfusion method could not only preserve better alveolar structure but also display perfect cellular antigen. Therefore, it is suitable for the morphometry-based researches on lung pathological and clinical diagnosis.