The alteration in intestinal secretory immunoglobulin A and its secreting cells during ischemia/ reperfusion injury / 解放军医学杂志

ABSTRACT

Objective To investigate the change in intestinal secretion immunoglobulin A (sIgA) level and IgA-secreting cells during ischemia/reperfusion (I/R) injury. Methods Forty-eight BALB/c mice were randomly divided into 6 experimental groups in accordance with different reperfusion times (R2h, R6h, R12h, R24h, and R72h group), and one sham group (n=8). Bacterial translocation to distant organs (lung, spleen, and mesenteric lymph nodes) was observed. The sIgA level of the intestinal tract was measured by enzyme-linked immunosorbent assay (ELISA). The B cell subgroup in the lymphocytes related to the intestinal tract was measured by flow cytometry. Results The bacterial translocation occurred during I/R injury, and the intestinal sIgA level decreased, and they showed an obvious negative correlation (r2=0.729). With the increase in intestinal I/R injury, the ratio of IgM+B220+ cells in the gut-associated lymphoid tissue increased, whereas the proportion of IgA+B220+ cells decreased. The most significant change was found in R12h group (P<0.01). Conclusions The proportion of IgM+ B cells in the gut-associated lymphoid tissue increased, whereas that of IgA+ B cells reduced during I/R injury. These phenomena may cause sIgA level to reduce and bacterial translocation of the distant organs to occur.