Application of plant medicine containing spermine in pingyangmycin fermentation / 中草药

ABSTRACT

Objective: Screening a plant medicine containing spermidine to promote the fermentation of pingyangmycin (PYM). Methods: Based on the screening method of carbon and nitrogen sources in biological fermentation, the plant medicine with the best promotion effect on PYM was screened out by HPLC method in Lycium ruthenicum, Lycium barbarum, Lycium barbarum bud, Carthamus tinctorius, and Coicis Semen. And the best adding time, amount and frequency were also screened out. Results: The results showed that Lycium barbarum bud had the best effect on PYM fermentation, and the initial adding time was 24 h, the adding interval was 24 h, and the adding frequency was three times. The shaking bottle fermentation level was 21.3 μg/mL when the addition amount was 7 g/L/per batch, and the 25 L tank fermentation level was 37.5 μg/mL when the addition amount was 18 g/L/per batch, which increased by 23.8% and 118.0%, respectively. Compared with the addition of spermidine, the yield was increased by 45.3%. Conclusion: Most plant drugs containing spermidine can promote the fermentation of pingyangmycin, and the selection of Lycium barbarum bud as raw materials for pingyangmycin fermentation meets the production requirements in terms of cost, yield and environmental protection.