Effects of butyl alcohol extract of Baitouweng Decoction on Candida albicans adhesion based on pH-mutant strains under acidic conditions / 中草药

ABSTRACT

Objective: To investigate the effect and mechanism of butyl alcohol extract of Baitouweng Decoction (BAEB) on adhesion of Candida albicans based on pH signaling pathway. Methods: Spot assay method was used to detect the sensitivity of pH mutants to BAEB under acidic conditions. XTT assay was used to detect the effect of BAEB on metabolic activity of pH mutants. The effect of BAEB on the adhesion activity of pH mutants was observed by fluorescence microscopy. The effect of BAEB on hydrophobicity of pH mutant was determined by n-octane inclusion method. The effect of BAEB on the expression of adhesion genes related to pH mutants was detected by qRT-PCR. Results: Under acidic conditions, spot assay observation showed that pH mutants were less sensitive to BAEB, 512 μg/mL BAEB interfered with pH mutants for 24 h and 48 h, there was no significantly decrease in bacterial colony. XTT assay showed that the metabolic activity of WT, PHR2 complementation, rim101/rim101 and RIM101 complementation was significantly inhibited in 512 μg/mL BAEB, and there was no significantly difference in the inhibition of phr2/phr2 metabolic activity. Fluorescence microscopy showed that the cell adhesion activity of WT, PHR2 complementation, rim101/rim101, RIM101 complementation was significantly inhibited in 512 μg/mL BAEB, the cell adhesion activity of phr2/phr2 had no obvious effect in 512 μg/mL BAEB. The n-octane inclusion method showed that the effect of 512 μg/mL BAEB on the cell surface hydrophobicity of WT, phr2/phr2, PHR2 complementation, rim101/rim101, RIM101 complementation was not significant. The qRT-PCR assay showed that the adhesion genes of pH mutants was inhibited in 1024 μg/mL BAEB. Conclusion: Under acidic conditions, the Candida albicans pH mutants was inhibited by BAEB to a certain extent.