Study on changes of active components in Lianzhifan solution under different storage conditions / 中草药

ABSTRACT

Objective: To study on changes of active components in Lianzhifan solution (LZF) under different storage conditions, which may provide a reference for the application and quality improvement of the LZF. Methods: HPLC analysis was performed on Eclipse Agilent C18 column (250 mm × 4.6 mm, 5 μm). The gradient elution was performed by the mobile phase consisting of acetonitrile and 0.1% formic acid aqueous with the flow rate of 1.0 mL/min, the detection wavelength was set at 238 nm, and the column temperature was 25 ℃. A fingerprint analysis method for the chemical composition of LZF was established to analyze the change of main active components with time under sealed and unsealed storage conditions. Results: The precision, stability, repeatability and sample recovery of the HPLC fingerprint of LZF were all in accordance with the requirements, and can be used for the analysis of the chemical constituents of LZF. The similarity values of sample fingerprints and control fingerprints at different time points under both storage conditions were greater than 0.95; The nine chemical components in LZF were identified by using the external labeling method and spectral characteristic analysis. The content of alkaloids (columbamine, epiberberine, jatrorrhizine chloride, coptisine chloride, palmatine chloride, and berberine) did not change significantly under the two storage conditions. However, the content of the iridoids, genipin-1-β-D-gentiobioside and geniposide was significantly reduced, and the content of genipin was increased in the first 3 months but decreased after 3 months. It is speculated that genipin-1-β-D-gentiobioside and geniposide can be converted to genipin by the enzyme in solution, and genipin may be degraded by other enzymes produced by microorganisms. Conclusion: During the six-month storage period, LZF still has a convert tendency, which may be caused by the action of enzymes produced by microbes. In order to improve the quality consistency of LZF product, it is recommended to adopt more scientific method to judge the endpoint and it is better to inactivate enzyme and sterilize after the end of the fermentation processing.