Establishment of HPLC fingerprint of Yuquan Pills and quantitative analysis of six components / 中草药

ABSTRACT

Objective: In order to provide a scientific basis for the quality control of Yuquan Pills (YP), HPLC fingerprint strategy was established and six components were determined. Methods: The HPLC analysis was performed on Symmetery C18 column (250 mm × 4.6 mm, 5 μm), using acetonitrile and 0.1% phosphoric acid solution as the mobile phase at a flow rate of 1.0 mL/min, and the column temperature was 30 ℃. The fingerprints of 11 batches of YP were established and evaluated by the similarity evaluation system of TCM (version 2012A), clustering analysis and principal component analysis. Furthermore, the content of puerarin, daidzein, verbascoside, schisandrin, glycyrrhizic acid, and glycyrrhizin was determined. Results: The HPLC fingerprint with 12 common peaks of YP was established, and the similarities of samples were over 0.9. After validating the multiple component quantitative analysis condition through methodology, the average recoveries (n = 9) were between 92.06% and 109.34%, and the RSD were in the range of 0.22%-2.76%. The content of puerarin, daidzein, verbascoside, schisandrin, glycyrrhizic acid, and glycyrrhizin in 11 batches of YP were in the range of 0.838-2.777, 0.550-1.014, 0.312-0.618, 0.023-0.092, 1.154-1.674, 0.035-0.052 mg/g, respectively. Conclusion: The combination methods of HPLC fingerprint and simultaneous determinations of multiple components are rapid, simple and reproducible, which can provide methodological reference for the quality control of YP.