Study on recovery of erythrocyte function in one-time exhaustive swimming rats by ginseng polysaccharide / 中草药

ABSTRACT

Objective: To investigate the effect of ginseng polysaccharide on the recovery of erythrocyte function in rats with one-time exhaustive swimming. Methods: Rat exhaustion swimming model was divided into control group, exhaustion group, self-recovery group after exhaustion, and low, middle and high dose groups of ginseng polysaccharide. The degree of oxidative damage of erythrocyte, ATP, ATPase, SA content in rat serum were detected by ELISA, and the activity of Na+, K+-ATPase and Ca2+, Mg2+-ATPase in rat red blood cells were detected. The level of Band 3 protein in erythrocyte membrane was detected by Western blot and changes in the activities of key enzymes of glycolysis caused by Band 3 protein were determined. Results: After exhaustive swimming, the red blood cells of rats were damaged by oxidative stress. The anti-oxidant capacity of red blood cells was significantly lower than that of the control group. The ginseng polysaccharide could promote the recovery of anti-oxidant capacity of red blood cells in a dose-dependent manner. The content of ATPase and SA were decreased compared with the control group. With the increase of ginseng polysaccharide concentration, the content of ATP, ATPase and SA were increased gradually. The activities of Na+, K+-ATPase and Ca2+, Mg2+-ATPase were decreased after exhaustive swimming. The ginseng polysaccharide can restore the function of sodium potassium pump and calcium magnesium pump in a dose-dependent manner. The content of Band 3 protein after exhaustive swimming was significantly lower than that of the blank control group. The ginseng polysaccharide can restore the content of erythrocyte membrane Band 3 protein. The activities of the key enzymes of glycolysis, PK, HK and PFK, were adjusted in a dose-dependent manner. Conclusion: Ginseng polysaccharide has a good recovery effect on rat erythrocyte function damage caused by exhaustive exercise.