Inhibitory effect and mechanism of Pien Tze Huang on proliferation, migration, and invasion of lung cancer cells / 中草药

ABSTRACT

Objective: To study the inhibitory effect of Pien Tze Huang on proliferation, migration, and invasion of lung cancer cells and explore its mechanism. Methods: Human lung cancer cell A549 was used as the experimental object in vitro, and the cells were treated with different concentrations of Pien Tze Huang. MTT was used to detect cell proliferation, and the changes of cell invasion and migration were measured by Transwell assay. Western blot was used to detect the phosphorylation of invasive migration proteins MMP-9, MMP-2 and EMT proteins E-cadherin, vimentin and Akt signaling pathway proteins. Lung cancer cells were treated with Akt signaling pathway activator and tablets to detect the effect of activating Akt signaling pathway on the proliferation, invasion, and migration of lung cancer cells. Results: Pien Tze Huang (100 μg/mL tablets) had no effect on the proliferation of lung cancer cells, the proliferative ability of lung cancer cells could be significantly reduced by 200 μg/mL Pien Tze Huang. The invasion and migration ability of lung cancer cells treated with 100 and 200 μg/mL tablets were decreased, the protein expressions of MMP-2 and MMP-9 in cells were decreased, the level of E-cadherin protein in cells was increased, the level of vimentin protein was decreased, at the same time, the phosphorylation level of Akt was decreased. Akt signaling pathway activator treatment could significantly reduce the inhibitory effect of Pien Tze Huang on proliferation, invasion and migration of lung cancer cells (P < 0.05). Conclusion: Pien Tze Huang decreased the proliferation, migration and invasion of lung cancer cells, the mechanism is related to the inhibition of the activation of Akt signaling pathway.