Network analysis and mechanism prediction of “effectiveness-toxicity” of liver injury in treatment of hepatitis with Xiaochaihu Decoction / 中草药

ABSTRACT

Objective: To explore the “efficacy-toxicity” targets, pathways, and mechanism of Xiaochaihu Decoction in the treatment of hepatitis based on the ADR information excavating of drug-induced liver injury in the treatment of hepatitis with Xiaochaihu Decoction. Methods: Taking Xiaochaihu Decoction as the research object, the integrated pharmacology platform was used to analyze and predict key targets and pathways. Cytoscape software was used to collate and analyze key targets and pathways to obtain co-participation pathways and mechanisms of “efficacy-toxicity”. Results: The analysis of the effect of Xiaochaihu Decoction for liver injury in the treatment of hepatitis showed that there were 40 common targets such as HADHA, HADH, NSDHL, ADH1A, ALDH 3A2, and GCK among the top 100 candidate targets with degree value. The chemical components jointly participated in the network of “effect-toxicity” which belonged to Xiaochaihu Decoction including 27 components in Bupleuri Radix, 74 components in Ginseng Radix, 13 components in Pinelliae Rhizoma, 8 components in Zingiberis Rhizoma, 32 components in Glycyrrhizae Radix et Rhizoma, 39 components in Jujubae Fructus, and 33 components in Scutellaria baicalensis. A total of 226 components were involved in the “effect-toxicity” process through endocrine and metabolic diseases, non-alcoholic fatty liver disease (NAFLD), estrogen signaling pathway, neurodegenerative diseases, nervous system, chemokine signaling pathway, and endocrine system. Conclusion: The “efficacy-toxicity” effect of Xiaochaihu Decoction on liver injury in the treatment of hepatitis may be closely related to Ras/Raf/MEK/ERK, NF-B, and PI3K/AKT signal transduction pathway. It plays a role by regulating cell proliferation, cell apoptosis and the expression of inflammatory factors, which provides a theoretical basis for further pharmacological and toxicological verification and chemical separation research. It plays a role by regulating cell proliferation, cell apoptosis and the expression of inflammatory factors.