Quality evaluation of steamed Panax notoginseng Powder based on partial least squares discriminant analysis / 中草药

ABSTRACT

Objective: To establish the method for the specific chromatograms analysis of steamed Panax notoginseng Powder and determine the content of eight components, combined with clustering analysis and partial least square discriminant analysis (PLS-DA), with aim to provide reference for the quality control of steamed Panax notoginseng Powder. Methods: HPLC with Agilent ZORBAX SB-C18 column (250 mm × 4.6 mm, 5 μm) was used, the mobile phase was acetonitrile (B)-water (A) in a gradient elution mode, the detection wavelength was set at 203 nm, the flow rate was 1.0 mL/min, the column temperature was 40 ℃, and sample size 10 ìL. HPLC characteristic spectrum of steamed Panax notoginseng Powder was established, and quantitative determination methods of eight index components, ginsenoside 20 (S)-Rh1, 20 (R)-Rh1, Rk3, Rh4, 20 (S)-Rg3, 20 (R)-Rg3, Rk1 and Rg5, were investigated, its content in 63 batches of samples were determined. Results: The specific chromatograms of steamed Panax notoginseng Powder effective parts were established and 19 common peaks were designated. Among them, eight rare saponins including ginsenoside 20 (S)-Rh1, 20 (R)-Rh1, Rk3, Rh4, 20 (S)-Rg3, 20 (R)-Rg3, Rk1 and Rg5 all showed good linear relationship within the ranges of 0.999 9, 0.999 5, 0.999 4, 0.999 3, 0.999 1, 0.999 3, 0.999 1, and 0.999 3, respectively. The average recovery was 95%—105%, with the RSD value less than 2%. Moreover, the 63 batches of samples were divided into two groups brown red and light yellow, and the quality of brown red group was obviously better than that of pale yellow group. Conclusion: The quality of the steamed Panax notoginseng Powder is related to the color and lustre. This method is accurate, sensitive and reproducible, which can provide reference for the quality evaluation of steamed Panax notoginseng Powder.