RP-HPLC-UV determination of seven chemical constituents and principal component cluster analysis of Dendrobium / 中草药

ABSTRACT

Objective: The RPLC-UV detection method of seven chemical components was established, and the difference of 21 species of Dendrobium were identified, with aim to provide reference for the quality control and resource development of Dendrobium. Methods: Agilent 1200 UPLC was performed on a XDB-C18 chromatographic column with the sample size of 5 μL, the flow phase was selected according to different chemical components, and SPSS20.0 software was used for principal component analysis and cluster analysis. Results: The composition and content of seven chemical compositions of 21 species of Dendrobium were significant. No erianin was detected in 10 kinds of species, and the content of erianin of D. chrysotoxum in the other 11 species was significantly higher than others, quercetin was undetected in 12 species, gallic acid was undetected in 11 species, vanillin was undetected in four species, naringenin was undetected in one specie, but syringic acid and coumarin were detected in all 21 species of Dendrobium. Among them, coumarin of D. heterocarpum was significantly higher than the others. According to the principal component analysis, 21 species of Dendrobium were scattered in 3D spatial distribution, and it could be grouped into six types at the distance of 5. Therefore, different Dendrobium had obvious differences in the composition and content of these seven chemical compositions, and different Dendrobium should be treated differently when using these seven components to characterize pharmacodynamic functions. Conclusion: The establishment of RPLC-UV method can provide reference for the quality control of Dendrobium and the development strategies of different germplasm resources.