HPLC fingerprint combined with chemical pattern recognition for integral quality of Shujin Huoxue products / 中草药

ABSTRACT

Objective: To establish the fingerprint chromatography of Shujin Huoxue products (Shujin Huoxue Capsule and Shujin Huoxue Tablet) and provide a reference for its overall quality evaluation. Methods: The analysis was performed on Agilent Poroshell 120 SB-C18 (150 mm × 4.6 mm, 2.7 μm) column with acetonitrile-0.2% formic acid solution for gradient elution, the flow rate was 0.5 mL/min, the detection wavelength was 277 nm, and the column temperature was 30 oC. HPLC of 23 batches of Shujin Huoxue products were analyzed by the software “Similarity Evaluation System for Chromatographic Fingerprint of TCM (2012 edition)” combined with principal component analysis (PCA), cluster analysis (CA), and orthogonal partial least squares discriminant analysis (OPLS-DA). Results: The similarity of 18 batches (S1-S17 and S23) of samples was more than 0.900, 23 batches of samples could be clustered into three groups by PCA, CA, and OPLS-DA analysis. Among these, S1-S16 were clustered into group I, S23 and S17 could be divided into group II, and S18-S22 were clustered into group III. Four common peaks with larger differences were determined from 10 common peaks, including peaks 1, 3 (5-hydroxymethylfurfural), 6 (phenylacetic acid), and 10 (4-methoxy salicylaldehyde). Conclusion: Fingerprint chromatography combined with chemical pattern recognition technique was stable and simple, which could be used to evaluate the quality of Shujin Huoxue products systematically and comprehensively.