Fingerprint and multi-components content determination of standard decoction of Angelicae Sinensis Radix / 中草药

ABSTRACT

Objective: To establish an HPLC method for the fingerprint analysis and content determination of standard decoction of Angelicae Sinensis Radix (ASR), and to provide an effective method to ensure the quality of standard decoction of ASR. Methods: Fingerprint of standard decoction of ASR was established by HPLC. The similarity evaluation combined with cluster analysis (CA), principal component analysis (PCA), and partial least squares discriminant analysis (PLS-DA) were applied to explore chromatographic peak of main affecting the quality of standard decoction of ASR, and to determine the contents of ferulic acid, ligustilide H, ligustilide I, ligustilide and tryptophan. All samples were analyzed by Kromasil C18 column (250 mm × 4.6 mm, 5 μm) maintained at 35 ℃ and eluted with acetonitrile-0.1% formic acid at the flow rate of 1.2 mL/min, and the detection wavelength was 280 nm. Results: There were 17 common peaks in the HPLC fingerprint of established standard decoction of ASR, and the similarity of 15 batches of standard decoction of ASR was between 0.788 and 0.983. The samples were broadly divided into three categories by CA and PCA. Seven markers were verified by PLS-DA, and peaks 8, 17, 10, and 12 were identified as ferulic acid, ligustilide, ligustilide I, and ligustilide H, respectively. In quantitative analysis, the five components showed good regression (r2 > 0. 999 0) with linear range, the content respectively was 0.041%-5.596% in ferulic acid, 0.026%-1.583% in ligustilide H, 0.201%-6.461% in ligustilide I, 0.126%-4.942% in ligustilide, and 0.481%-2.753% in tryptophan, and the average recoveries were in the range of 99.43%-104.35%. Conclusion: The analysis method established in this experiment is stable, reliable, and repeatable, which can provide reference for the quality evaluation of standard decoction of ASR.