Cloning and expression analysis of ATP/ADP transporter protein gene PgAATP1 in Panax ginseng / 中草药

ABSTRACT

Objective: To clone the full-length cDNA of the ATP/ADP transporter protein (AATP) genes in Panax ginseng to provide resources and some knowledge necessary for the further gene function study. Methods: The mRNA sequence of the AATP genes in other plants were downloaded on the website of NCBI and used to perform local Blast alignment in the transcriptome of Jilin ginseng from 14 tissues. The AATP gene in Panax ginseng was cloned by PCR, and analyzed using bioinformatics software and online resources. The expression pattern of PgAATP1 gene in 14 tissues of Panax ginseng was analyzed using the expression profile of transcriptome and its expression level under methyl jasmonate was deceted by quantitative real-time PCR. Results: A full-length cDNA sequence was successfully cloned from Panax ginseng and named as PgAATP1, which was 1866 bp in length and encoded 621 amino acids. The relative molecular mass of PgAATP1 protein calculated was 67 897.23, and the isoelecric point calculated was 9.58. It was found that the protein was similar to the plastid AATP in other species. The expression of this gene was high in all tissues but higherin fruit flesh and leaf blade, and the expression of PgAATP1 gene was up-regulated by methyl jasmonate. Conclusion: We have obtained the full-length of PgAATP1 gene. This gene expressed higher in tissues of vigorous starch synthesis and responsing to methyl jasmonate.