Simultaneous determination of 16 chemical components in Psoraleae Fructus based on quantitative analysis of multi-component with single-marker / 中草药

ABSTRACT

Objective: The purpose of this study was to establish a QAMS analytical method for 16 compounds in Psoraleae Fructus, and attempt to evaluate the quality difference among different batches of Psoralen Fructus by chemometrics. Methods: All experiments were performed on three different HPLC instruments. Isopsoralen was used as the internal reference substance to determine the relative correction factors of the other 15 compounds. The robustness and durability of the measured relative correction factors of the 15 compounds were evaluated on different chromatograph instruments and columns; And the measurement result deviation was compared between QAMS method and the external standard method. Results: Under the established chromatographic conditions, the relative correction factors of 15 compounds in Psoraleae Fructus had high accuracy, good durability, and good reproducibility under different experimental conditions. The results obtained from two analysis methods showed no significant deviation. The results obtained by the new established QAMS analytical method showed that the consistency of compound types among different batches of Psoraleae Fructus were better, but the content of different componounds was relatively different. Conclusion: The newly established QAMS analytical method for simultaneous determination of 16 compounds in Psoraleae Fructus provides a more efficient method to evaluate the comprehensive quality of Psoraleae Fructus from different sources.