Spectrum-effect relationship of wind-dispersing and exterior syndrome-relieving of Shufeng Jiedu Capsules / 中草药

ABSTRACT

Objective: To explore the potential effective compounds of ‘wind-dispersing and exterior syndrome-relieving’ of Shufeng Jiedu Capsule (SJC). Methods: The fingerprints of 22 samples with different proportions of SJC were established by LC-MS. The stimulation rates of 22 samples to the M3 receptor were measured by Chinese hamster ovarian cancer cells with high expression of M3 acetylcholine receptor which related to ‘wind-dispersing and exterior syndrome-relieving’. Finally, the spectrum-effect relationship of relative peak areas of characteristic peaks and stimulation rates was analyzed by BP-neural network. Results: According to the established optimal neural network model, 16 characteristic peaks were calculated and found to be significantly correlated with the activity of SJC on the M3 receptor stimulation. Conclusion: Through the spectrum-effect study, it is speculated that the effective components of SJC on ‘wind-dispersing and exterior syndrome-relieving’ may be the 16 compounds including dihydrodylate glycoside, emodin, rhein, phillyrin, forsythoside E, saikosaponin d, polydatin, verbascoside, etc, which provides a basis for the determination of quality markers.