Simultaneous determination of 10 active ingredients in Yinzhihuang Oral Liquid by HPLC / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 3648-3653, 2019.
Article
in Chinese
| WPRIM
| ID: wpr-850955
ABSTRACT
Objective:
To establish an HPLC method to simultaneously determine 10 active ingredients in Yinzhihuang Oral Liquid (YOL) and provide scientific basis for the quality control, evaluation and standard revision of Yinzhihuang preparations.Methods:
An HPLC-UV method was used with a Dikma Diamonsil C18 column (200 mm × 4.6 mm, 5 μm). The mobile phase was acetonitrile-0.1% acetic acid solution with gradient elution (0-15 min, 8%-12% acetonitrile; 15-40 min, 12%-30% acetonitrile; 40-55 min, 30%-60% acetonitrile; 55-75 min, 60%-35% acetonitrile; 75-80 min, 35%-8% acetonitrile). The detection wavelength was 240 nm. The flow rate was 1.0 mL/min. The column temperature was 30 ℃. The injection volume was 20 μL.Results:
Ten active ingredients (neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, geniposide, p-hydroxyphenylacetone, scutellarin, baicalin, quercetin, baicalein and wogonin) in YOL were simultaneously determined. The linearity was good (r ≥ 0. 999 0), the limit of detection and quantification were 0.003-0.018 μg/mL and 0.009-0.055 μg/mL. The average recoveries were 99.7%-102.6% with RSDs of 0.34%-6.14%. The average content of the above 10 ingredients was in turn (0.21 ± 0.09), (0.47 ± 0.01), (0.87 ± 0.06), (4.71 ± 0.27), (0.94 ± 0.20), (4.52 ± 0.80), (41.75 ± 3.53), (9.85 ± 1.67), (0.45 ± 0.09), (3.51 ± 0.89) mg/mL. The content of baicalin and geniposide was 35.44-45.82 mg/mL and 4.16-4.92 mg/mL in eight batches, respectively. All eight batches of YOL meet the requirements of the Chinese Pharmacopoeia 2015 with qualified in quality.Conclusion:
The established HPLC method is simple, specific, sensitive, stable, precise, accurate, and reproducible, which can be used for quality control and evaluation of YOL.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional and Herbal Drugs
Year:
2019
Type:
Article
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