Genetic diversity of Forsythia suspensa based on ISSR / 中草药

ABSTRACT

Objective: To investigate the genetic diversity of Forsythia suspensa from different populations. Methods: ISSR markers were used to analyze the genetic diversity of 25 populations of F. suspensa. Percentage of species level polymorphic bands (P), Nei’s genetic diversity index (H), and Shannon’s information index (I) of genetic information were calculated by POPGEN 32. UPGMA relationship dendrogram was clustered by NTSYS. Results: Thirteen primers produced 353 bands and P was 100%. H and I were 0.252 3 and 0.394 0, and genetic differentiation coefficient (Gst) and gene flow (Nm) were 0.331 8 and 1.007 0 within the population levels. The genetic distance varied from 0.031 0 to 0.155 5. NTSYSpc software was used to cluster the system. A total of 25 clusters were divided into two categories and seven groups. Cluster analysis was conducted among Forsythia individuals. A total of 195 samples were divided into two categories and five groups. Conclusion: The genetic diversity among the populations of F. suspensa is at higher level. However, the genetic distance among the Forsythia population is not related to the geographical distance, which is mainly dependent on the ecological factors and the growth environment.