Chemical constituents from rhizomes of Cimicifuga dahurica / 中草药

ABSTRACT

To isolate and identify the chemical constituents from the rhizome of Cimicifuga dahurica. Methods The isolation and purification of 60% EtOH extract of the rhizomes of C. dahurica were carried out through various modern chromatographic separation techniques HP-20, silica gel, ODS, Sephadex LH-20 column and semi-preparative HPLC. And the structures of the compounds were identified based on spectroscopic data and physicochemical properties. Results Twenty compounds were isolated and identified as cimicifugaside F (1), (+) (2S,3R)-2-(4-hydroxy-3-methoxyphenyl)-3-[(β-D-glucopyranosyloxy) methyl]-7-methoxybenzofuran-5-propenoic acid (2), 5-hydroxy-2-methoxybenzoic acid (3), benzoic acid 4-O-β-D-glucoside (4), isoferulic acid (5), ferulic acid (6), trans-ferulic acid 4-O-β-D-allopyranoside (7), trans-ferulic acid 4-O-β-D-glucoside (8), (E)-sinapic acid 4-O-β-D-glucoside (9), 6,6’-di-O-sinapoylsurcose (10), piscidic acid (11), fukinolic acid (12), N-trans-feruloyltyramine 4-O-β-D-allopyranoside (13), N-trans-3’-methoxy-4’-feruloyltyramine-4-O-β-D-allopyranoside (14), N-trans-3’-methoxy-4’- feruloyltyramine-4-O-β-D-glucoside (15), grevilloside G (16), (-)-syringaresinol (17), (-)-syringaresinol 4,4’-di-O-β-D- allopyranoside (18), (+)-isolarisiresinol 3a-O-β-D-glucoside (19), (-)-5’-methoxyisolariciresinol 3a-O-β-D-glucoside (20). Conclusion Compound 1 was identified as a new lignan, and compounds 2-4, 8-10, 15-17 and 20 were isolated from Cimicifuga genus for the first time.